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In the sport of artistic roller skating the athletes use shoes fitted with small wheels to use in rinks and consists of seven disciplines characterized by different physical demands. Roller skaters are judged on content and manner of performance, and this includes the skater’s ability to do jumps, spins, and footwork. The purpose of this study was to compare the body composition, flexibility, balance, reactive strength, maximum strength, and aerobic capacity based on skating discipline and skating level. A total of 108 female athletes from the three individual disciplines (figures, freestyle, and solo dance) and three different levels (elite, sub-elite, and non-elite) volunteered to participate in this study. Findings showed that freestyle skaters have less body fat, and higher levels of reactive and maximum strength than figures and solo dance skaters. Elite skaters demonstrate greater values of flexibility, balance, strength, and aerobic capacity compared to their sub-elite and non-elite counterparts. The results of this study showed that there are differences between elite and non-elite athletes and between skating disciplines. Strength and conditioning professionals should have that into consideration when training these athletes.