Effect of melamine feeding on the ovastacin localization and protein level in ovulated eggs.

(A) Ovastacin was stained with rabbit polyclonal anti-mouse ovastacin antibody and examined by confocal microscopy. Scale bar, 20μm. (B) Measurement of fluorescent intensity of ovastacin signals. There was no significant difference of ovastacin signals between control (n = 15) and treatment (n = 15) groups. Data were presented as mean percentage (mean ± SEM) of at least three independent experiments. Asterisk denotes statistical difference at a P