HepG2 Cell : Negative control vs miR-224 vs miR1912 transfected

Microarray analysis was performed by transfection of miR-224 and miR-1912 to HepG2 cells to identify the genes regulated by miR-224 and miR-1912. miR-224 is known to down-regulate the mRNA of PCSK9, and miR-1912 was also predicted to down-regulate PCSK9 and verified in our previous experiments. PCSK9 was closely associated with the development of hyperlipidemia by interfering with the recycling of LDLR, and the decrease in PCSK9 and the increase in LDLR gene expression were greater in cells treated with miR 1912. HepG2 cells were cultured on DMEM supplemented with 10% FBS. 2 × 10 5 cells were plated on 1 well of 12-well cell culture plate . miR-224 and miR-1912 were purchased from Ambion Inc, and transfection was performed with Lipofectamine 2000 following the manufacturer's instrument. Then the transfected cells were cultured in DMEM supplemented with 10% FBS and 1% P/S for 24 hours and media was changed to DMEM supplemented with 10% DLPS and 1% P/S. After the day, transfected cells were harvested and RNAs were perpared with miRNeasy micro kit (Qiagen). Analysis was performed for control, miR-224, miR-1912 treated HepG2.