Identifying Zmat3-bound RNAs
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下载链接：
https://www.ncbi.nlm.nih.gov/sra/SRP250021
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资源简介：
The goal of the study was to identify RNAs bound by Zmat3 using eCLIP. Overall design: Three lines of sgNC Cas9+ E1A;HrasG12V MEFs and one Zmat3 knockout MEF sample expressing E1A and HrasG12V were used for the experiment. Briefly, 20 million cells were UV crosslinked, lysed, sonicated, and treated with Rnase. Then the samples were immunoprecipitated with anti-Zmat3 antibodies. After washes, samples were run on a NuPAGE gel, transferred to nitrocellulose membrane, and the region from 35 to 110 kDa was isolated and treated with Proteinase K. RNA was isolated, reverse transcribed, and sequenced on an Illumina HiSeq4000.
创建时间：
2020-11-07
