Left Atrial Cardiomyocyte Transcriptomic Analysis of Postoperative Atrial Fibrillation

After IRB approval and informed consent, cardiomyocytes were isolated from human left atrial tissue samples obtained along the standard atriotomy incision during mitral valve surgery. RNA was isolated from cardiomyocytes and RNA libraries were prepared after rRNA depletion. RNA sequencing and whole transcriptome analysis were performed to identify differential gene expression associated with new onset postoperative atrial fibrillation.]]>
Inclusion Criteria Adult participants with either 1) no previous history of Atrial Fibrillation (AF) or 2) persistent AF presenting for elective mitral valve surgery for severe degenerative mitral regurgitation.Exclusion CriteriaParticipants with prior cardiac surgery, mitral stenosis, aortic regurgitation, aortic stenosis, and endocarditis were excluded. ]]>
Participants were enrolled between April 2021 and June 2022.]]>