Immune cell senescence and exhaustion
收藏科学数据银行2023-11-03 更新2026-04-23 收录
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资源简介：
Initially, we conducted wound healing tests on HCT116 cells, employing SDC1 or SDC4 knockdown. The extent of wound healing was assessed at 0h, 24h, and 48h, and wound healing percentages were calculated using ImageJ software (v1.54d). Subsequently, Transwell migration assays were carried out to evaluate the migratory capacity of HCT116 cells after the knockdown of SDC1 or SDC4. Finally, q-PCR was conducted to measure the expression levels of EMT-related markers E-Cadherin (CDH1), N-Cadherin (CDH2), and Vimentin (VIM) in HCT116 cells following siRNA knockdown of SDC1 and SDC4.
提供机构：
Lisheng Peng; Guangzhou University of Chinese Medicine
创建时间：
2023-11-02
