Sequencing of ShCAST RNA-guided in vitro transposition products
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https://www.ncbi.nlm.nih.gov/sra/SRP324004
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资源简介:
Total DNA sequencing of pools of in vitro transposition products in plasmid (pTarget_CAST) using the ShCAST Tn7-CRISPR-Cas transposition system. Investigating role of TnsC ATPase in target-site selection through use of non-hydrolyzable ATP analog and mutant alleles. Mechanistic insights are relevant to genome editing applications.
创建时间:
2021-07-16



