the lncRNA expression profiles in fetal liver, adult liver and liver cancer tissues from mice

A large number of oncofetal molecules were found through expression profiling of a total of lncRNAs(35923)+coding genes(24881) from 17.5-day-old embryonic livers (three independent replicate samples named A1, A2, and A3), 2-month-old adult male mouse livers (three independent replicate samples named B1, B2, and B3) and one-year-old male mouse liver cancer tissues (three independent replicate samples named C1, C2, and C3) using a microarray analysis. To identify some oncofetal transcripts that potentially affect the occurrence and development of HCC, we established a DEN-induced mouse liver cancer model to obtain experimental samples considering the individual differences in human HCC samples, which are referred to as liver cancer in the following narrative. The liver cancer tissues were subjected to haematoxylin-eosin staining (HE staining) and verified according to their cell morphology under a microscope. Then, we analyzed the expression profiles of lncRNAs and protein-coding RNAs in 17.5-day-old embryonic livers (three independent replicate samples named A1, A2, and A3), 2-month-old adult male mouse livers (three independent replicate samples named B1, B2, and B3) and one-year-old male mouse liver cancer tissues (three independent replicate samples named C1, C2, and C3) using a microarray analysis.