Whole-genome resequencing of Pseudomonas aeruginosa PAO1 after anti-virulence treatments targeting iron uptake. Whole-genome resequencing of Pseudomonas aeruginosa PAO1 after anti-virulence treatments targeting iron uptake

We carried out an experimental evolution in human serum as an ex-vivo model and screened evolved lines for the evolution of resistance phenotypes towards two anti-virulence treatments, gallium and flucytosine, which both target the iron scavenging pyoverdine of Pseudomonas aeruginosa (each at 2 different doses). We performed whole-genome sequencing of 16 evolved clones from the different treatment regimes .