Structural and Molecular Analysis of Adult Mouse Astrocytes and Vascular Connectivity in the Cortex and Hippocampus

After image acquisition (0-RAW_CL230331_E2_serie1) and deconvolution (1-Deconvolved_CL230331_E2_serie1) using confocal microscopy and the SVI Huygens software,respectively, the image processing was conducted using Imaris, Fiji, and Matlab software. This process involved a sequence of manual operations (2-Imaris_surfaces_CL230331_E2_serie1) and custom Groovy scripts (5-Groovy scripts).

The dataset analysis (3-Imaris_final_CL230331_E2_serie1_ims) allowed for a deeper investigation of morphological and molecular properties of adult mouse astrocytes (4-Image analysis_CL230331_E2_serie1) in two brain regions, the Isocortex and the Hippocampus, known to be interconnected to support multiple cognitive functions.