Plant microRNA profiling of amiRNAs transgenic lettuce. Lactuca sativa

We conducted two amiRNA (amiR417 and amiR519) transgenic lettuce lines. In order to confirm that the target artificial miRNAs (amiR417 and amiR519) were correctly transcribed and analysis the cleavage sites of the 3’ and 5’ ends of amiR471, small RNA fragments were extracted from ten independent positive T2 generations and examined using Solexa deep sequencing. Overall design: Total RNA from 10 regular or T3 generation expressing amiR471 or amiR519 lettuce plants were extractedfor Solexa deep sequencing