Identification of differentially expressed genes influenced by NCOA6 in 22Rv1 human prostate cancer cells

In this study, we aimed to characterize the suppressive role of NCOA6 in prostate cancer development, uncover the underlying molecular mechanism, and identify potential therapeutic targets for treating NCOA6 loss-induced prostate cancer. Gene expression microarray analysis and RNA-Seq analysis were performed to identify differentially expressed genes influenced by NCOA6 in 22Rv1 human prostate cancer cells and mouse prostate tumors, respectively. Pathway enrichment analysis and gene set enrichment analysis were performed to identify NCOA6-regulated signaling pathways, particually the cancer-associated pathways. ChIP-Seq analysis was further performed to identify NCOA6-associated genomic regions in 22Rv1 cells. The potential direct target genes of NCOA6 were identified by combined analysis of the gene expression profiling data and ChIP-Seq data. Finally, we identified EGFR as one of the 264 NCOA6 direct target genes and deomonstrated that NCOA6 suppressed prostate cancer progression by preventing EGFR overexpression. Gene expression profiles in the 22Rv1Par., 22Rv1Ctrl, 22Rv1NCOA6KO #2-74, 22Rv1NCOA6KO #2-96, 22Rv1NCOA6KO #4-91 and 22Rv1NCOA6KO #4-105 cells cultured in RPMI-1640 complete medium were measured at 24 hours after cell passage. Eight samples were analyzed, including two 22Rv1Par. sample replicates and two 22Rv1Ctrl sample replicates that were used as controls as well as four different 22Rv1NCOA6KO cell clones.