Gene expression regulation by angiotensin II in late gestation fetal sheep. Ovis aries

We undertook a transcriptome-wide discovery approach to provide information on the involved gene regulatory responses to change in fetal cardiac mass in response to chronic infusion of angiotensin II or losartan. Overall design: One of each of 8 twin fetus pairs was infused with either angiotensin II or losartan daily for five days in utero. A portion of the left ventricular free wall of treatment and control fetuses was excised, homogenized in a reagent, and then total RNA was then isolated and assayed on the agilent microarray