Evasion protein expression in human cancer cell lines and stably expressing CHO transfectants.

PI-9, PD-L1 and EpCAM expression was determined by FACS analysis, Bcl-2 and IDO expression was assessed by Western blot and subsequent analysis with Image J software, and TGF-β and IL-10 were detected with specific ELISA assays. The expression level observed in the CHO EpCAM transfectants was set to 100%. Stimulation of cells with IFNγ was at 1000 U/ml for 48 h.an.d. = not determined.Evasion protein expression in human cancer cell lines and stably expressing CHO transfectants.