Sensitivity and specificity of gene methylation at optimal cut-off values for detection of colon cancer tissue.

TaqMeth V is expressed as mean��SD, and TaqMeth V is described in Materials and Methods.P value was derived from Wilcoxon matched-pairs signed-ranks test.aPT vs. PN.*P was considered significant.P value was derived from the Wilcoxon-Mann-Whitney test.bPT vs. NN.AUROC is expressed as mean��SD, and optimal cut-off values were calculated from ROC analysis.cP value in ROC analysis (PT vs. NN).dP value in Fisher's exact test (PT vs. NN).Sensitivity, positive methylation/total tumor cases (PT); Specificity, negative methylation/total normal cases (NN).% Methylation in PN was based on cut-offs from PT vs. NN.Methylation level below the cut-offs was considered as unmethylated and over the cut-offs were as methylated.PN, corresponding normal colon mucosa from CRC patients; PT, CRC tissues; NN, colon normal epithelium from non-cancer patients.