Soil metagenome

In August 2017, we collected rice root samples and rhizospheric soil for 5 replicates per plot (three replicates for CK), randomly collected 5-8 sub-samples and fully mixed the sub-samples to create a composite sample. The roots at a 20-30 cm depth were collected and shaken until soil no longer fell off. Then, the rhizosphere soil was carefully detached from the roots with a brush . Backup soil was stored at -80°C.