Microarray data from BM-MSCs exposed to DMSO, LG268, Rosi, TPhP, TBT, and MEHP. Microarray data from BM-MSCs exposed to DMSO, LG268, Rosi, TPhP, TBT, and MEHP

Primary mouse bone marrow mesenchymal stromal cells (BM-MSCs) cultured for bone differentiation were exposed to vehicle (DMSO), synthetic RXR ligand (LG100268), type 2 diabetes therapeutic and PPARγ ligand (Rosiglitazone), and environmental PPARγ ligands (Tributyltin, Triphenyl Phosphate, and MEHP) to evaluate differential gene expression as well as nuclear receptor expression and downstream PPARG target gene expression between all chemical exposures Overall design: Primary mouse BM-MSCs were isolated and induced to undergo osteogenesis. At initial differentiation stage, the primary cell cultures are treated with either the vehicle (DMSO) or test chemicals. Total RNA from samples were isolated 1 day or 4 days after treatment/differentiation began and hybridized on Affymetrix microarrays. There are 4 replicates of each experimental condition.