Effects of temperature treatments on cytosine-methylation profiles of diploid and tetraploid plants of the alpine species Ranunculus kuepferi (Ranunculaceae)

The current dataset refers to the DNA methylation patterns of diploid and
tetraploid individuals of Ranunculus kuepferi, obtained with the method of
methylation-sensitive AFLPs (MS-AFLPs). The individuals of Ranunculus
kuepferi were collected from several locations throughout the distribution
of the species in the Alps, transferred to the old Botanical Garden of
Göttingen and placed into two climate chambers MC1000E (Snijders
Scientific, Tilburg, Netherlands), where the temperature treatment
experiments took place. In the first chamber a cold treatment was applied
(+7°C day/+2°C night; frost treatment: -1°C cold shocks for three nights
per week), while in the second chamber a warm treatment was applied (+15°
day/+10°C night). The plants were shifted from one treatment to the other
one after the end of the 2016 flowering period and leaf material was
collected during the flowering period of 2016 and 2017. This material went
through the respective lab procedures in order to obtain the genome-wide
patterns of epigenetic variation via MS-AFLPs. The analysis of the
electropherograms was conducted with Peakscanner v.2 and fragment scoring
was performed with RawGeno 2.0-1 R package. These fragment scoring binary
matrices are presented here.