Quantitative Analysis of erythroblast cells Transcriptomes treated with cholesterol or not

In our work, we explored the dynamics of cholesterol homeostasis during terminal erythropoiesis. Interestingly, we found differential requirements of cholesterol during terminal erythropoiesis, with that cholesterol is essential for the early stage erythroblasts proliferation, while the downregulation of cholesterol biosynthesis is required for late stage cell cycle exit and final enucleation. To reveal the role of cholesterol in the the early stage erythroblasts proliferation and the detail mechanisms of erythroblasts late stage cell cycle exit, we performed RNA sequencing to analyze the gene expression profiles change between untreated and cholesterol treated erythroblasts. TER119-negative fetal liver erythroblasts were purified and cultured in Epo containing medium for 30 h supplemented with or without cholesterol (40 μM), and total RNA was extracted using the TRIzol reagent. RNA sequencing was conducted using the BGIseq500 platform (Shenzhen, China) in triplicate.