Strategic delivery of Omega-3 fatty acids for modulating inflammatory neurodegenerative diseases

Objectives: Early-life inflammatory events like infections and injuries may predispose the brain to Alzheimer's disease (AD) by disrupting neurodevelopment and raising vulnerability. The association between early neuroinflammation and subsequent neurodegeneration leading to dementia remains unclear. We hypothesize that omega-3 (n-3) fatty acids (FA), especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), positively regulate neuro-immune cells, preserving their cell membrane structure and metabolic homeostasis. Our study examined whether strategic delivery of n-3 FA via injectable n-3 triglycerides (TG) can influence microglial lipid metabolism to prevent or delay AD progression.
Methods and results: We characterized n-3 treatment effects on modulating lipid and metabolic homeostasis in microglia during the critical window of brain development. Our preliminary studies on determining the effects of early n-3 treatment on brain cell homeostasis indicate that perinatal bolus..., , , # Strategic Delivery of Omega-3 Fatty Acids for Modulating Inflammatory Neurodegenerative Diseases

Data for the effect of n-3 fatty acids on inflammatory neurodegenerative diseases. The experiment was located in New York, USA.

Contact Dr. Chuchun L. Chang ([[e-mail]](mailto:[e-mail])) with any questions.

Description of the data and file structure:

Quantitative Real-time PCR

Total ribonucleic acid (RNA) was isolated from the whole brain using Trizol reagent (Invitrogen). Single-stranded complementary deoxyribonucleic acid (cDNA) was synthesized using iScript reverse transcriptase (Bio-Rad). Relative quantification of messenger ribonucleic acid (mRNA) expression was determined by real-time qPCR with Fast SYBR Green PCR kit (Applied Biosystems) normalized to a housekeeping gene GAPDH on Thermal Cyclers (Bio-Rad). The difference between the target gene and the housekeeping gene Ct value (ΔΔCt) was calculated for all experiments. The expression of the targe...,