Binding of ATR:ATRIP to RPA at resected DNA DSBs

ATR (ATM- and rad3-related) kinase is an essential checkpoint factor in human cells constitutively associated with ATRIP (ATR-interacting protein). The ATR:ATRIP complex binds RPA complex (RPA1:RPA2:RPA3) associated with ssDNA at resected DNA double-strand breaks (DSBs). Complex formation is primarily mediated by physical interaction between ATRIP and RPA1 (Zou and Elledge 2003, Jazayeri et al. 2006).

ATR（ATM-和rad3相关）激酶是人类细胞中构成性关联于ATRIP（ATR相互作用蛋白）的关键检查点因子。ATR:ATRIP复合物与切割DNA双链断裂（DSBs）处结合单链DNA（ssDNA）的RPA复合物（RPA1:RPA2:RPA3）相结合。复合物的形成主要是由ATRIP与RPA1之间的物理相互作用所介导（Zou和Elledge，2003年，Jazayeri等人，2006年）。