Examining Pathogenesis in Genome Instability Associated Neurodegenerative Mouse Model [TotalStranded-RNA-seq]

To investigate the molecular basis for ataxia in the DNA repair deficient mice we utilized RNA-seq to evaluate the transcriptome of AtmNes-cre;Aptx-/- (and AtmNes-cre;Parp1-/-) cerebellar and cortical tissue. Using total RNA from symptomatic 10-month-old double-mutant mice and littermate controls, we performed the transcriptome analysis. top 1,000 expressed genes in each genotype Total Stranded RNA sequencing of the cerebellum and cortex examining gene expression in ATM APTX null and associated control brains.为探究DNA修复缺陷型小鼠共济失调的分子机制，我们采用RNA测序（RNA-seq）对AtmNes-cre;Aptx-/-（及AtmNes-cre;Parp1-/-）小鼠的小脑与大脑皮层组织的转录组进行分析。我们使用出现症状的10月龄双突变小鼠及其同窝对照的总RNA，完成了转录组分析。本研究针对各基因型中表达量排名前1000的基因，对小脑与大脑皮层开展了链特异性RNA测序（Total Stranded RNA-seq），以检测ATM、APTX双敲除及其相关对照脑组织的基因表达水平。