Drop assay after fresh transformation.
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Yeast co-transfected with either pSos-Nef, pSos-Vpr or pSos-CA or pSos (empty vector) and either pMyr-VHH19 (an anti-Nef sdAb previously described), pMyr-aCA2 (selected by phage display), pMyr (empty), pMyr-SB (Sos binding protein), or pMyr plasmids coding for Vpr1A, Vpr2A, CA7A or CA8B were resuspended and spotted onto galactose or glucose plates. Growth of colonies on galactose plates was indicated by + or - according with number and size of colonies. Drop assay after fresh transformation.
创建时间:
2014-12-01



