RNA-seq of Histamine stimulated HUVECs infected with shNT or shH1R

To investigate the mechanism of histamine H1 receptor (H1R)- mediated proliferation and angiogenesis of endothelial cells, mRNA profiles of HUVEC cells transduced with shNT or shH1R under histamine treatment for 9 hrs were generated by deep sequencing, in replicate, using Illumina GAIIx. We identified the upregulation of genes in several pathways including angiogenesis, proliferation and migration that could be responsible for the increased tube formation in histamine treated shNT expressing HUVEC cells mRNA profile of shNT or shH1R expressing HUVEC cells under histamine treatment for 9 hrs were generated by deep sequencing, in replicate, using Illumina HiSeq 2500 .