Targeted metabolomics data for dimethyl-ribityl lumazine (DMRL) and riboflavin in Mycobacterium tuberculosis and Mycolicibacterium smegmatis mutants of the riboflavin biosynthetic pathway

This dataset contains raw mutliple reaction monitoring (MRM) data for intermediate metabolites of the riboflavin biosynthetic pathway (RBP) in wild-type and riboflavin biosynthetic pathway gene deletion mutants of Mycobacterium tuberculosis and Mycolicibacterium smegmatis. These strains were generated to investigate the source of metabolic antigens recognized by mucosal-associated invariant T (MAIT) cells, which detect intermediates from the riboflavin biosynthetic pathway. To determine the impact of these gene deletions on the production of riboflavin and its precursors, we developed an MRM assay for DMRL and Riboflavin. This dataset provides evidence of how these disruption impact the biosynthesis of DMRL and Riboflavin which has enables a better understanding of the source of MR1 antigen in mycobacteria., , , # MRM assay - Mycobacterium tuberculosis (DMRL and Riboflavin)

Dataset DOI: [https://doi.org/10.5061/dryad.z08kprrr1](https://doi.org/10.5061/dryad.z08kprrr1)

## Description of the data

This dataset contains data that contributes to key finding on the study of metabolic antigens recognized by MR1 restricted T cells in Mycobacteria. The data provided includes targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of riboflavin biosynthetic pathway mutants of Mycobacterium tuberculosis and Mycolicibacterium smegmatis. The dataset provides qualitative and quantitative analysis of Dimethyl-ribityl Lumazine and Riboflavin in the generated mutants. All LC-MS/MS data have been provided in the original format allowing processing with free and accessible tools. 

#### Growth and preparation of Mtb and Msm for metabolomics

Glycerol stocks of Mtb and *Msm* strains were used to start cultures on 7H11 agar plates supplemented with the appropriate antibiotics with or without ..., ,