Preliminary experiments to assess the suitability of DLS for protein interaction studies with huntingtin-HAP40 2019/08/12

<strong>Project: </strong>Biophysical investigation of the HTT interactome <strong>Experiment: </strong>Preliminary experiments to assess the suitability of DLS for protein interaction studies with huntingtin-HAP40 <strong>Date completed:­ </strong>2019/08/12 <strong>Rationale: </strong>HTT is a multi-valent protein interactor and scaffold. It is likely that many of the proteins HTT interacts with form weak or transient interactions. One method which may be fruitful to detect this type of interaction is dynamic light scattering – a method which allows in solution calculation of protein particle radius. HTT-HAP40 Q23 (https://zenodo.org/record/3383265), PACSIN1 (https://zenodo.org/record/3256589) and PFN1A (kindly donated by Opher Gileadi and Tracy Keates) were analysed in a dilution series by DLS to assess signal to noise and concentration dependency of protein radius and polydispersity.