CO1 sequences used to compare the performance of five taxonomic assignment programs

A test of the accuracy of taxonomic assignments of some CO1 sequences was built into the technical validation process of MIDORI2. We removed 186 complete mitochondrial CO1 gene sequences, one from each of the 186 taxonomic classes represented in MIDORI2, from the LONGEST_GB242 CO1 database to use as queries.
Accuracy tests were conducted for three regions of the query sequences: 1) the complete mitochondrial CO1, 2) the ~658 bp COI “barcode region” (Folmer et al., 1994), and 3) a ~313 bp fragment (Leray et al., 2013) within the barcode region.
This file contains the 186 CO1 sequences that were used as query to compare the performance of five taxonomic assignment programs (Qiime2, Mothur, RDP classifier, Sintax and BLAST+)