Next Generation Sequencing Facilitates Quantitative Analysis of MV4-11 cell line Transcriptomes

Purpose: The goals of this study are to compare side population and main population cells transcriptome profiling (RNA-seq) . Methods: We sorted side population and main population cells by staining Hoechst 33342 and using Flow Cytometer (Beckman Coulter, Moflo XDP). 200,000 cells per sample were analysed in RNA-Seq experiments.The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays Overall design: 4 samples: CTR-SP,CTR-MP,A+H-SP,A+H-MP.A+H:AC220+HHT treated MV4-11 for 24h.