File S1 - Directed Differentiation of Patient-Specific Induced Pluripotent Stem Cells Identifies the Transcriptional Repression and Epigenetic Modification of NKX2-5, HAND1, and NOTCH1 in Hypoplastic Left Heart Syndrome

File contains Figure S1 and Tables S1 and S2. Figure S1. Verification of inhibitory effects of shRNAs for NKX2-5, HAND1, and NOTCH1 in CPCs. (A) HLHS-derived CPCs were cultured in growth medium and transfected with transcriptional factors as indicated with or without corresponding four sets of shRNAs. The inhibitory effects for each gene were confirmed by real-time RT-PCR. (B) The most efficient shRNA for each gene was selectively used to inhibit endogenous expression of transcription factors in BV-derived CPCs. Full length of cDNA for each transcription factor was transfected into HLHS-derived CPCs and the repressive effect was examined. Data were obtained from more than five-independent experiments and normalized by using β2-microglobulin and human heart tissue for comparisons. *, pTable S1. Expression of embryonic development-associated genes in patient-derived CPCs and their iPS cell derivatives during reprogramming. Table S2. Primers used for RT-PCR, quantitative RTPCR, bisulfite sequencing analysis, and ChIP assay. (DOC)