An optimized RNA-seq workflow for isolating single nuclei from clinical biopsies

We developed an optimized protocol for nuclei isolation from small core needle biosies taken from both healthy and diseased human kidney samples Overall design: Profiled the single nuclei transcriptome of 5 kidney allograft biopsies histologically characterized as normal/nonspecific (N=1), acute tubular necrosis (N=2), and chronic antibody mediated rejection (N=2)