Prehabilitation as a biologically active intervention reprograms pancreatic tumor-immune microenvironment to enhance antitumor immunity

This dataset contains NanoString GeoMx Digital Spatial Profiling data generated from surgically resected pancreatic ductal adenocarcinoma (PDAC) specimens collected from patients who underwent a multimodal prehabilitation program and matched untreated controls. Spatial transcriptomic profiling was performed across immune (CD45 positive), tumor (PanCK positive), and stromal regions of interest to investigate the biological effects of prehabilitation on the PDAC tumor microenvironment. Differential expression, pathway enrichment, and immune deconvolution analyses were used to evaluate transcriptional changes associated with cytotoxic immune activation, stromal remodeling, and oncogenic signaling. The goal of this exploratory dataset is to provide an initial resource for investigating how prehabilitation may influence immune–tumor interactions in PDAC and to support future studies integrating biological and clinical endpoints. Overall design: Formalin-fixed paraffin-embedded pancreatic ductal adenocarcinoma (PDAC) tissues were obtained from patients who participated in a multimodal prehabilitation program prior to surgical resection and from matched control patients who underwent standard management. Spatial transcriptomic profiling was performed using the NanoString GeoMx Digital Spatial Profiler with whole-transcriptome probes. Regions of interest were selected based on immunofluorescent segmentation markers to capture immune (CD45 positive), tumor (PanCK positive), and stromal compartments within each specimen. Raw DSP count matrices were generated for each compartment and used for downstream differential expression, pathway enrichment, and immune deconvolution analyses. The dataset was generated as part of an exploratory pilot study to examine the biological effects of prehabilitation on the tumor microenvironment in PDAC.