Small RNAseq analysis of C.elegans from Parental P0 to F3 generations under hypoxic stress in both WT and hrde-1 mutant [small RNA-seq]

The goal of small RNA-seq is to identify the differentially expressed small RNAs (microRNA, 22G RNA, piRNA, snoRNA) in the wild-type (WT) and hrde-1 mutant of C.elegans at the normoxia reared and hypoxia treatment conditions at P0 generation, and those small RNAs maintained dysregulation in F1, F2 and F3 generation. Overall design: Total RNA from WT and hrde-1 under normoxia or hypoxia treatment worms from P0 to F3 generations were extacted, and 32 small RNA libraries were built using the Nextflex Small RNA-seq Kit v3 (NOVA-5132-06), in duplicate for each condition, using single-end 85 bp on Illumina Illumina Nextseq 500 platform