Effect of RAS gene mutation in acute myeloid leukemia

RAS gene mutation is prevalent in AML, and the RAS signaling pathway is closely related to many metabolic pathways. However, the effects of different RAS gene mutations on AML cell metabolism are unclear.Ba/F3 is an IL-3-dependent mouse pre-B cell line. It can survive independently of IL-3 after the introduction of driving mutant gene.We used Ba/F3 cell line to prepare a cell model of NRAS(Q61K) and KRAS(G12V) mutation by lentivirus transfection to study the effect of RAS gene mutation.