The P-body protein 4E-T regulates the balance between cell genesis and establishment of the postnatal neural stem cell pool by repressing mRNA translation [4ET_neurosphere]

To investigate the targets of 4E-T in postnatal V-SVZ precursors cultured as neurospheres We performed RNA immunoprecipitations (RIPseq) on neurosphere lysates with anti-4E-T or control anti-IgG antibodies, isolated and sequenced the resulting RNA Overall design: Neurospheres from the V-SVZ of P7/P8 animals were cultured for 7 days, and immunoprecipitations were performed using anti-4E-T or anti-IgG control antibodies, and the associated mRNA was isolated