Mu transposon insertion sites in matched leaf and endosperm samples from Mu-active W22 maize

Mu transposon insertions were sequenced in the parents and offspring from 4 maize families using MuSeq2. Male parents were Mu-active while female parents were Mu-inactive, and so all new transposon activity can be associated with the male parent. Matched endosperm and leaf from each offspring were sequenced, allowing inherited insertions to be unambiguously identified as these tissues diverge prior to fertilization (each is derived from a sister sperm cell in pollen during double fertilziation). The data were analyzed to follow the transmission of de novo Mu insertions from parent to offspring. Matched endosperm and leaf tissues were collected from Mu-active plants. Endosperm samples were first obtained by seed chipping; then the chipped seed was grown to collect V2 seedling leaves. Plants were Mu-active, generated by crossing a Mu-active male parent to a Mu-inactive female parent, both of the W22 maize inbred background