Zebrafish_eye_populations_transcriptomics. Zebrafish_eye_populations_transcriptomics

The zebrafish eye develops from a neuroepithelial layer of retinal progenitors, which then become postmitotic and differentiate into the mature retinal cell types. After 72hpf, the embryonic development of the retina is completed, since all major cell types are present and arranged in the characteristic lamination of the vertebrate retina. At this point, the cells located in the central retina have already exited the cell cycle and almost all growth comes from a stem cell niche located at the periphery of the retina, called the ciliary marginal zone (CMZ). The CMZ is an heterogeneous region composed of stem cells, which continuously supply the CMZ with intermediate progenitors, that gradually proliferate and differentiate moving towards the central retina, a process that spatially recapitulates the temporal progression of embryonic development. The aim of this project is to characterise the transcriptome of the stem cells and the proliferative progenitors in order to build gene networks within each compartment, and subsequently characterise the mechanisms by which a stem cell gives rise to a differentiated cell in a post-embryonic retina This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/