CellPPD-Mod: Prediction of Cell-Penetrating Potential of Modified Peptides Containing Natural and Chemically Modified Residues

CellPPD-Mod is an advanced computational resource designed to predict the cell-penetrating potential of peptides that contain both natural and chemically modified residues. While most existing tools are restricted to peptides composed of the 20 standard amino acids, CellPPD-Mod addresses a critical gap in the field by accounting for the diverse chemical modifications—such as non-natural residues, D-amino acids, and fatty acid attachments—commonly used to enhance the stability and delivery efficiency of therapeutic peptides. Web Server: https://webs.iiitd.edu.in/raghava/cellppdmod/ Citation Kumar, V., Agrawal, P., Kumar, R., Bhalla, S., Usmani, S. S., Varshney, G. C., & Raghava, G. P. S. (2018). Prediction of Cell-Penetrating Potential of Modified Peptides Containing Natural and Chemically Modified Residues. Frontiers in Microbiology, 9:725. https://doi.org/10.3389/fmicb.2018.00725 About the Research Chemical modifications are often essential to prevent the rapid degradation of peptides by proteases in the body. However, these modifications can significantly alter a peptide's ability to cross cell membranes. CellPPD-Mod provides a systematic method to evaluate these "hybrid" sequences. Extensive Dataset: The models were developed using a comprehensive dataset of 1,273 modified peptides (949 CPPs and 324 non-CPPs) curated from the CPPsite 2.0 database. Chemical Diversity: The dataset includes peptides with various modifications, including D-amino acids, unusual amino acids, and terminal modifications like acetylation or amidation.