Single-cell RNA-seq of hiPSCs under 5iLAF naïve culture conditions

We applied single-cell RNA sequencing, to define heterogenous cell populations present in human naïve 5iLAF cultures. Noteworthily, although Epiblast-like cells represented the main cell population, we detected a population with a trophectoderm-like gene expression profile which could be subclustered according to three different stages of early trophectoderm maturation. Furthermore, we provided evidence for the existence of an 8-cell-like cell population, equivalent to the 8-cell human embryo. Thus, the 5iLAF naïve culture condition emerges as an excellent system to model gene regulation in the earliest stages of human embryogenesis, including zygotic genome activation.. Overall design: Single-cell RNA-seq of hiPSCs (G15.AO) cultured under 5iLAF naïve conditions