Next Generation Sequencing Analysis of Gene Profiles of Healthy, Hyperlipidaemia, Familail Hyperlipidemia, Coronary Heart Disease and Familial Coronary Heart Disease

To explore genes indicating a risk of familial (premature) coronary heart disease (FH-CHD) development in familial hypercholesterolaemia (FH), 30 Thai male volunteers were enrolled: 7 healthy controls (N), 6 patients with hypercholesterolaemia (H), 4 with FH, 10 with CHD, and 3 with FH-CHD. Transcriptome data were investigated using next-generation sequencing analysis in whole blood (n = 3). Genes that were significantly expressed in both FH and FH-CHD, but not in N, H, and CHD groups, were selected, functionally analysed, and validated by quantitative real-time PCR (RT-qPCR). We identified 55 intersecting genes between FH and FH-CHD groups. The expressions of intersecting genes that shared between FH and FH-CHD groups are potential for further development as predictive markers for FH-CHD in FH patients.