Evaluation of VapC models.

VapC structural models were evaluated by QMEAN4 and Z score in order to compare and rank alternative models of the same target. QMAN4 is a reliability score consisting of a combination of four structural descriptors which ranges between 0 and 1 with higher values for better models. Z-score provides an estimate of the “degree of nativeness” of the structural features observed in a model; ‘good-quality’ models reach a mean Z-score of −0.65, ‘medium-quality’ -1.75, and the ‘low-quality’ −3.85. The analysis of the model quality scores showed that Shigella VapC is the only template to render a “good” model for VapC from Leptospira (*), as “good” as the one created for Salmonella VapC (**), which shares 89% identity with the template. In opposition, Mycobacterium VapC20 model (#) displayed low scores.