Hamm and Gray 2024 Figure 5

<b>FIG 5 Strains lacking polyP and (p)ppGpp have disrupted cell division.</b><b> </b>(<b>A</b>)<b> </b>Confocal fluorescence time-lapse microscopy of the mutant <i>ppk relA spoT</i><i> </i>FtsZ-GFP (MJG2405) on an LB agarose pad at 37°C. The triple mutant forms two Z-rings in the middle of the cell, releasing a mini cell (red arrows). There are also two Z-rings which form at either pole of a single cell, both functional and releasing a mini cell (white arrows). Example of mini-cell formation and release can be seen in CEM (S4). (<b>B</b>) Confocal fluorescence time-lapse microscopy of the mutant <i>ppk relA</i><i> </i>FtsZ-GFP (MJG2403) on an LB agarose pad at 37°C. This image shows a mutant forming three Z-rings at one pole, and at least 3 at the opposite pole as well, with no Z-rings forming in the middle of the cell, for a total of six Z-rings in a single cell. This cell continued to grow without lysing (<b>Supplemental Video SV5</b>).