RNA-seq profiles of B cells and naive and activated T cells from human peripheral blood

Mature B cells, CD4+ T cells and CD8+ T cells were isolated from the peripheral blood of two human donors. Naive CD4+ and CD8+ T cells were cultured for three days in the presence of Dynabeads Human T-Activator CD3/CD28 beads (Life Technologies, cat. no. 111.31D) to induce activation. The B cells and naive and activated T cells were profiled by whole transcriptome RNA-seq. Overall design: Cells were isolated from two human donors, resulting in two biological replicates of each combination of cell type and activation status.