Mutations in FMDV M296I passaged in the presence of ribavirin.

aThe sequence of the entire viral genome was determined for FMDV M296I after 2, 5, 8 and 10 passages in the presence of 5 mM ribavirin. The populations are those described in Fig. 1B. Mutation and deduced amino acid substitutions are relative to the sequence of the parental clone FMDV M296I.bFMDV genomic region analyzed; residue numbering is according to [74].cMutations and deduced amino acid substitutions found. Amino acid residues (single-letter code) are numbered individually for each protein from the N- to the C-terminus. Boldface type indicates a change in the amino acid residue. Two residues separated by a bar indicate a mixture of two nucleotides in the population, according to the sequence band pattern. Procedures for nucleotide sequencing and identification of FMDV genomic regions are described in Materials and Methods.