Genome-wide maps of the androgen receptor, pS213 androgen receptor, and 14-3-3 ? chromatin occupancy in control and PIM1 over-expressing LNCaP cells

We used ChIP-seq to identify chromatin binding sites for AR, 14-3-3 ?, and pS213 AR in the absence and presence of PIM1 over-expression. We find that, as expected, pS213 AR occupancy almost completely overlaps with total AR occupancy, and increases in the presence of PIM1 over-expression. Additionally, the majority of pS213 AR sites are co-occupied by 14-3-3 ?. 14-3-3 ? is a known AR co-activator, but this study definitively shows its co-occupancy on chromatin with AR and pS213 AR. Overall design: Examination by ChIP-seq of AR, pS213 AR, and 14-3-3 ? in control LNCaP and PIM1 over-expressing LNCaP cells.