Supplementary materials for "Proteomic profiling of bone tissue reveals distinct pathways in men and women with osteoporosis"

Figure S1. PCA of female and male osteoporostic and control groupsA.PCA of female osteoporostic (green circle) and control groups (red circle)B.PCA of male osteoporostic (green circle) and control groups (red circle)PCA, principal component analysisFigure S2. Protein-protein interaction network analysis of key differential protein for women and menA.Key differential protein interaction network analysis for female osteoporosis-control comparison groupB.Key differential protein interaction network diagram for male osteoporosis-control comparison groupCircles represent proteins, and lines represent the associations between proteins.Figure S3. Immunohistochemistry analysis of COX7A2 expression in women (30×)A. Immunohistochemically stained sections of bone tissue in women with osteoporotic fractures (n=3)B. Immunohistochemically stained sections of bone tissue in women with violent fractures (n=4)C. There was no difference in the AOD values of COX7A2 between osteoporotic fractures and violent fractures in women. Statistical significance was assessed by a 2-tailed independent <i>t</i> test.AOD, Average optical densityFigure S4. Immunohistochemistry analysis of HLA-C expression in men (10×)A. Immunohistochemically stained sections of bone tissue in men with osteoporotic fractures (n=3)B. Immunohistochemically stained sections of bone tissue in men with violent fractures (n=3)C. There was no difference in the AOD values of HLA-C between osteoporotic fractures and violent fractures in men. Statistical significance was assessed by a 2-tailed independent t test.AOD, Average optical densityFigure S5. Immunohistochemistry (10×) analysis of ITGB2 expression in menA. Immunohistochemically stained sections of bone tissue in men with osteoporotic fractures (n=3).B. Immunohistochemically stained sections of bone tissue in men with violent fractures (n=3).C. There was no difference in the AOD values of ITGB2 between osteoporotic fractures and violent fractures in men. Statistical significance was assessed by a 2-tailed independent t test.AOD, Average optical densityTable S1. Fracture sites in female and male patients with osteoporotic or violent fracturesTable S2. Correlation of female and male core DAPs with BMD and BTMs (adjusted by age)