five

mutation site of Arabidopsis thaliana mutant crc1-1

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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA488909
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F2 plants generated from backcrossing crc1-1 with Col-0 plants were inoculated with CMV-2aT∆2b at the concentration of 50 μg/mL. Genomic DNA were extracted from about 100 susceptible F2 plants using CTAB reagent. DNA libraries were constructed according to standard Illumina protocols and used for SNP analysis by next-generation sequencing with illumina HiSeq2000 platform. The wild type genomic DNA was sequenced as a reference.
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2018-09-02
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