Table S1 - CRISPR Interference Directs Strand Specific Spacer Acquisition

Integrated spacer sequences. PIM: Plasmid interfering mutant, Spacer #: spacer number, corresponds to spacer locations in Fig. 1 and Fig. 3. PAM: protospacer adjacent motif, non-consensus PAMs are underlined. Target: Location of protospacer on pRSF-1b (Kan: Kanamycin resistance gene. Ori: RSF1030 origin of replication. Bb: pRSF-1b backbone, lacI: lac operon repressor gene). Target position: Nucleotide position of the spacer match (protospacer) on pRSF-1b. Spacers matching protospacers on the (+) and (-) strand are coloured red and black, respectively. Note that the actual targeted strand during CRISPR interference by Cascade is the complementary strand of what is indicated here.
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