Table 1 in Purification and characterization of Bowman-Birk and Kunitz isoinhibitors from the seeds of Rhynchosia sublobata (Schumach.) Meikle, a wild relative of pigeonpea

Table 1 Purification of trypsin-specific protease inhibitors (RsPI) from mature seeds of R. sublobata. RsBBI and RsKI were separated from RsPI as described in Fig. 3A and their specific activity and purification fold were determined.
Purification stepTotal protein (mg)Total activity (TI units)Yield recovery (%)Specific activity (TI units/mg protein)Purification foldCrude protease inhibitors1014.433600010035.481.00Ammonium sulfate fractionation (20–60%)477.42968482.4562.171.75Affinity chromatography51.361525042.36296.928.36Gel filtration chromatography (RsPI)9.0996427.671107.1131.20RsBBI4.26758421.061780.2850.16RsKI1.089042.51837.0323.59