Peripheral RNA biomarkers for neurocognitive impairment shared by prenatal alcohol exposure (PAE) and offspring from mother with diabetes (OMD) in mice.

Our study reports peripheral RNA biomarkers for neurocognitive impairment shared by PAE and OMD in mice. We extracted RNA from three subtypes (T cell, B cell and monocytes) of white blood cells sorted by flow cytometer. We performed both differential expression and splicing analyses. Common differential alternative splicing (AS) events between OMD and FASD were found. This study posits that AS of lymphocyte RNA is a rich resource, and deep-learning is an effective tool, for discovery of peripheral biomarkers of neurobehavioral deficits in children of diverse adverse pregnancies. Overall design: RNA profiling extracted from peripheral blood mononuclear cells (PBMCs) of offspring from PAE, OMD and controls