Targeting an engineered DNA fragment to a specific site in chromosomes in order to disrupt, overexpress or modify the nucleotide sequence of a gene requires homologous recombination repair mechanism.
Deletion of biosynthetic gene clusters relevant for production of chrysogine, roquefortine and fungisporin/related tetrapeptides in Penicillium chrysogenum DS68530 (∆hdfA, ∆Pen-BGC).
